Journal: Frontiers in Cellular and Infection Microbiology
Article Title: CD147 mediates the adsorption of influenza A virus on the cell surface through direct interaction with HA
doi: 10.3389/fcimb.2025.1647283
Figure Lengend Snippet: Virus infection, adsorption and glycosylation modified form of CD147. (A) DF1 cells were infected with H5N6 (JX) virus (MOI 0.001), and the cells were harvested and analyzed the change of protein level via WB. (C) A549 cells were infected with H1N1 (PR8) virus (MOI 0.01), and the cells were harvested and analyzed the change of protein level via WB. (E) A549 cells were exposed to PD1530356h, 6h 12h and 24h with different concentrations, and infected with PR8(MOI 10) at 4°C for 1h, the cells were harvested and analyzed the change of protein level via WB. (G) MDCK cells were exposed to PD1530356h 24 hours, and infected with H9N2 (MOI 10) at 4°C for 1h, the cells were harvested and analyzed the change of protein level via WB. (B, D, F, H) Gapdh was used as the control, Image J(NIH) was used to quantitatively analyze the gray level of protein bands, and CD147/Gapdh indicated the relative level of CD147. All comparisons were tested by two-tailed Student’s t test, the solid line represents the difference of HG-CD147 in different infection periods, and the dotted line represents the difference of LG-CD147 in different infection periods, blue * represents the difference of LG-CD147 in the same infection period, red * represents the difference of HG-CD147 in the same infection period, and black * represents the difference of total protein of CD147. *, P, 0.05; **, P, 0.01; ***, P, 0.001; ****, P, 0.001.
Article Snippet: The following antibodies were used: anti-Flag mouse monoclonal antibody (Sigma, Germany, #F1804); anti-CD147 rabbit monoclonal antibody, anti-CD147 mouse poly-clonal antibody, anti-HA rabbit polyclonal antibody, and anti-GAPDH mouse mono-clonal antibody (Wuhan Sanying, #11989-1-AP, #66443-1-Ig, #51064-2-Ig, and #60004-1-Ig, respectively); anti-Strep mouse monoclonal antibody (AbClon, #AE066); and rabbit polyclonal antibodies against IAV proteins HA, NA, M1, M2, and NP (GeneTex, #GTX127357, #GTX125974, #GTX125928, #GTX640415, and #GTX125923, respectively).
Techniques: Virus, Infection, Adsorption, Glycoproteomics, Modification, Control, Two Tailed Test
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